Lecture 1 fundamentals of protein chemistry and mass spectrometry. Also explore the seminar topics paper on mass spectrometry with abstract or synopsis, documentation on advantages and disadvantages, base paper presentation slides for ieee final year chemical engineering chem or ieee chemical btech, be, mtech students for the year 2015 2016. This is the first of a pair of videos intended to help senior school chemistry students with mass spectrometry. Eggers elaborate the plan for a timeofflight mass spectrometer after a principle proposed by w. When students ask what books to read on mass spectrometry, there are various excellent monographs, but the ideal textbook still seemed to be missing at least in my opinion. These lecture notes were first drafted in dutch by peter van galen in 1992, when the only mass spectrometer in the faculty of science was the departments. Mass spectrometry 30 references 31 3 mass spectrometry 33 3.
Sep 20, 20 mass spectrometry works by ionizing chemical compounds to generate charged molecules or molecule fragments and measuring their mass tocharge ratios. Introduction definition classification atomic ms molecular ms principle of ms instrumentation ion sources mass analyzers detectors vacuum system applications 2 introduction. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. Tokmakoff lecture 42017, 1011, lobby suite, university union, 1st floor todays lecture. It is a histogram usually acquired using an instrument called a mass spectrometer. Aston, nobel lecture 1922 we learned to make elephants fly. Lecture 1 basics of mass spectrometry 1 mass spectrometry. Ppt mass spectrometry powerpoint presentation free to. Electronimpact ionization sample inlet 107 108 torr rh electron beam 70 ev 6700 kjmol. Also explore the seminar topics paper on mass spectrometry with abstract or synopsis, documentation. A mass spectrum plural spectra is a plot of the ion signal as a function of the masstocharge.
Native mass spectrometry on the intact protein level allows also for the analysis of molecules which rely on noncovalent interactions to preserve critical structural. When ionization occurs most the ions do not even enter the mass spectrometer. Lecture notes organic structure determination chemistry. Nov 24, 2015 this is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. Mass spectrometry is the study of matter through the formation of gasphase ions that are detected and characterized by their mass and charge. The number of hits may also be reduced by taking an accurately measured. William old fall 2011 2002 nobel prize in chemistry for developing electrospray ionization john fenn, virginia commonwealth university electrospray ionization for mass spectrometry of large biomolecules j. In mass spectrometry we work with charges and lenses electromagnetism. The separation chamber of the mass spectrometer is keep under a high vacuum and so all.
Esi is usually coupled in separation systems which are fed with slow ions. A plot of relative abundance of ions vs mz of all charged particles is the mass spectrum. Separation and analysis of the fragments provides information about. Mass spectroscopy ppt mass spectrometry time of flight. Fragmentation mechanisms in electron impact mass spectrometry. Lecture 1 introduction, history, scope and terminology of. The ions then fragment into smaller pieces, which are magnetically sorted according to their mass tocharge ratio mz. Definitions of terms relating to mass spectrometry iupac.
Applications of mass spectrometry in proteomics article pdf available in australian journal of chemistry 667 july 20 with 1,573 reads how we measure reads. Lecture 1 fundamentals of protein chemistry and mass. Thomson discovers the electron and determines its mz nobel prize in 1906 1912 j. Learn simple and easytounderstand lcms approaches for structure identification of metabolites, impurities, natural products, and biomolecules.
These will be considered separately and then followed with a series. Hello everybody, welcome to week three of the coursera. Apr, 2012 this is the first of a pair of videos intended to help senior school chemistry students with mass spectrometry. Separation and analysis of the fragments provides information. Mass spectrometry ms is an analytical technique that measures the mass tocharge ratio of ions. Analysis and interpretation of mass spectral data american. High resolution mass spectrometry of antibody drug. Mass spectrometric methods for analyzing glycoproteins and glycans mass spectrometry in an omics world me. Mass spectrometry seminar report, ppt, pdf for chemical.
Molecular weight structure background the impact of a stream of high energy electrons causes the molecule to lose an electron forming a radical cation. Introduction mass spectrometry ms is an analytical technique that measures the masstocharge particles. History of mass spectrometry 1942 first commercial sector mass spectrometer by cec. In person analysis and interpretation of mass spectral data. Tokmakoff lecture 42017, 1011, lobby suite, university union, 1st floor.
Pdf applications of mass spectrometry in proteomics. O connor powerpoint ppt presentation free to view mass spectrometry market mass spectrometry ms is a systematic technique that ionizes chemical species and classified the ions on their mass tocharge ratio. Lecture 1 fundamentals of protein chemistry and mass spectrometry arthur moseley arthur. Mass spectrometry for small organic molecules the mw can be determined to within 5 ppm or 0. Xxxx difference of 2 1 ppm this means the mass of one electron 0. Mass spectrometry is a gas phase technique the sample must be vaporized.
Mass spectrometry mass spectrometry refers to that branch of analytical science devoted to. Guest lecture protein and peptide mass spectrometry. Mass spectrometry is a destructive method used to measure molecular weight and provide. High resolution mass spectrometry of antibody drug conjugates using the orbitrap mass analyzer. Lecture 3 mass spectrometry principles of mass spectrometry mass spectrometry separates molecules based on the mass to charge ratio. In smallmolecule mass spectrometry, molecules are first ionized by collision with a highenergy electron beam. This lecture 1 include pesticides classification, introduction to mass spectrometry, vacuum system for agilent gc ms ms and ab sciex lc ms ms. Mass spectrometry ms is an analytical technique that measures the masstocharge ratio of ions. Compatible with a broad range of compounds fg pg sensitivity qualitative information mw up to 100,000 daltons or more with 0. Spectrometry background mass spectrometry mass spec or ms uses high energy electrons to break a molecule into fragments. High resolution mass spectrometry of antibody drug conjugates. Mass spectroscopy mass spectroscopy is a technique causing the formation of the gaseous ions with or without fragmentation.
The number of hits may also be reduced by taking an accurately measured isotope pattern of the ion into consideration. In smallmolecule mass spectrometry, molecules are first ionized by collision with a highenergy electron. William old fall 2011 2002 nobel prize in chemistry for developing electrospray. Lecture 1 introduction, history, scope and terminology of mass spectrometry. Hence, the mass spectrum of a sample is a pattern representing the distribution of ions by mass more. Owing to the rapid expansion of the field in the intervening years, particularly in mass spectrometry of biomolecules, a further revision of the recommendations has become necessary. Mass spectrometry for polymers benefits and analytical considerations for ms analysis of polymers the benefit of mass spectrometry is the ability to make absolute mass measurements at a.
Tandem mass spectrometry msms, ms2 or tandem mass spectrometry tandeminspace can be performed on ion beamtransmitting hybrid instruments with 2 distinct mass analysers. Pharmacy, pune a seminar on mass spectrometry as per pune university syllabus 1 st semester presented by. This lecture 1 include pesticides classification, introduction to mass spectrometry, vacuum. Mass spectrometry works by ionizing chemical compounds to generate charged molecules or molecule fragments and measuring their mass. Aston constructs the first mass spectrometer that allows focusing of ions by velocity nobel prize 1922. The results are typically presented as a mass spectrum, a plot of intensity as a function of the mass tocharge ratio. Mass spectrometry has spread by discipline as illustrated. Still the sensitivity is quite good and can allow the identification of proteins from.
This is a lecture and the second video takes you through past paper questions. The results are typically presented as a mass spectrum, a plot of intensity as a function of the massto. Mass spectrometry not part of electromagnetic spectrum what is measured are typically positively charged ions under certain circumstances negative ions can be detected once ions are in detector. Mass spectrometry is a powerful analytical technique used to quantify known materials, to identify unknown compounds within a sample, and to elucidate the structure and chemical properties of different molecules. Hence, the mass spectrum of a sample is a pattern representing the distribution of ions by mass more correctly. This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. Goldstein discovers anode rays in a gas discharge tube. Mass spectrometry works by ionizing chemical compounds to generate charged molecules or molecule fragments and measuring their mass tocharge ratios. Lecture notes in eimass spectrometry by torben lund. Introduction mass spectrometry ms is an analytical technique that measures the mass tocharge particles. Exact mass measurements remember that the mass measurement is determined for the ion and not the neutral molecule. It is used for determining masses of particles, for determining the elemental composition of a sample or molecule. Mass spectrometry and proteomics lecture 1 newcastle university. Mass spectroscopy ppt free download as powerpoint presentation.
The complete process involves the conversion of the sample into gaseous ions, with or without fragmentation, which are then. Thomson scanned the magnetic field and used electrical detection. Some important elements with monoisotopic masses in dalton, rounded to six digits. Explore mass spectrometry with free download of seminar report and ppt in pdf and doc format. Monoisotopic mass is typically expressed in unified atomic. He observes negative ions, multiply charged ions and identifies isotopes of 20ne and 22ne. O connor powerpoint ppt presentation free to view mass spectrometry. Find materials for this course in the pages linked along the left.